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Part:BBa_I716332:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-07-11)

ThpB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 29
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 29
    Illegal NotI site found at 343
    Illegal NotI site found at 786
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 29
    Illegal XhoI site found at 1070
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 29
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 29
    Illegal NgoMIV site found at 197
    Illegal NgoMIV site found at 821
    Illegal AgeI site found at 963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 77
    Illegal BsaI.rc site found at 668


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

ThpB
From IGEM07
PCR ThpBF/ ThpBIR on ThpB (610 bp, gp=A)
PCR ThpBR/ ThpBIF on ThpB (712 bp, gp=B)



PCR ThpBAF/ThpBAR on A+B (1285bp BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
Product pBca9145-BBa I716332


ThpBF Forward BglII site anneals to ThpB ctagaAGATCTgtgaccatcaccccgcccgc
ThpBIF Removing XhoI site in ThpB ggttcgagcggctcctTgaggaccagggctccggac
ThpBIR Removing XhoI site in ThpD gtccggagccctggtcctcAaggagccgctcgaacc
ThpBR Reverse XhoI site anneals to ThpB cgtcactcgagtcaggccgtttcgcggacgc


References